Intracellular coupling of bikunin and the heavy chain of rat pre-α-inhibitor in COS-1 cells
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چکیده
Pre-α-inhibitor is a serum protein consisting of two polypeptides : bikunin of 16 kDa, which carries an 8 kDa chondroitin sulphate chain, and heavy chain 3 (H3) of 74 kDa. The two polypeptides are linked through an ester bond between an internal Nacetylgalactosamine residue of the chondroitin sulphate chain and the C-terminal aspartic acid residue of H3. Both bikunin and H3 are synthesized by hepatocytes and become linked as they pass through the Golgi complex. H3 is synthesized with both N-
منابع مشابه
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1274 Proteins of the Inter - α - inhibitor Family Biosynthesis , Plasma Clearance and Interaction with Extracellular Matrix Components BY ANETA KACZMARCZYK
Kaczmarczyk, A. 2003. Proteins of the Inter-α-inhibitor family. Biosynthesis, Plasma Clearance and Interaction with Extracellular Matrix Components. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1274. 59 pp. Uppsala ISBN 91-554-5672-3 Bikunin, a chondroitin sulfate-containing protein of 25 kDa, has protease inhibitory activity and occurs in the plasma in free and...
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متن کاملPositive Mode LC-MS/MS Analysis of Chondroitin Sulfate Modified Glycopeptides Derived from Light and Heavy Chains of The Human Inter-α-Trypsin Inhibitor Complex*□S
The inter-α-trypsin inhibitor complex is a macromolecular arrangement of structurally related heavy chain proteins covalently cross-linked to the chondroitin sulfate (CS) chain of the proteoglycan bikunin. The inter-αtrypsin inhibitor complex is abundant in plasma and associated with inflammation, kidney diseases, cancer and diabetes. Bikunin is modified at Ser-10 by a single low-sulfated CS ch...
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تاریخ انتشار 1997